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Vladimír PUCHART, PhD
Research Scientist

Department of Polysaccharide Enzymology

Tel.: +421-2-59410239
Fax: +421-2-59410222
Research Interests
Carbohydrate biochemistry and enzymology, utilization of plant cell wall polysaccharides and oligosaccharides, regulation of synthesis, purification, characterization and mechanistic investigation of carbohydrate-active enzymes, reverse reactions of glycoside hydrolases and esterases associated with plant cell wall polysaccharide degradation, bioconversion of hemicelluloses
Publications
Puchart V., Katapodis P., Biely P., Kremnický L., Christakopoulos P., Vr¹anská M., Kekos D., Macris B. J., Bhat M. K.:
Production of xylanases, mannanases, and pectinases by the thermophilic fungus Thermomyces lanuginosus.
Enzyme Microb. Technol. 24, 355361 (1999)
Puchart V., Vr¹anská M., Bhat M. K., Biely P.:
Purification and characterization of Pgalactosdiase from a thermophilic fungus Thermomyces lanuginosis.
Biochim Biophys. Acta - General Subjects 1524, 2737 (2000)
Biely P., Puchart V., Cote G. L.:
Enzymic Egalactosylation of a cyclic glucotetrasaccharide derived from alternan.
Carbohydr. Res. 332, 299303 (2001)
Puchart V., Vr¹anská M., Svoboda P., Pohl J., Ögel Z. B., Biely P.:
Purification and characterization of two forms of endo-i1,4mannanase from a thermotolerant fungus, Aspergillus fumigatus IMI 385708 (formerly Thermomyces lanuginosus IMI 158749).
Biochim. Biophys. Acta - General Subjects 1674, 239250 (2004)
Puchart V., Biely P.:
Glycosylation of internal sugar residues of oligosaccharides catalyzed by Ggalactosidase from Aspergillus fumigatus.
Biochim Biophys. Acta - General Subjects, 1726, 206-216, (2005)
Biely P., Puchart V.:
Recent progress in the assays of xylanolytic enzymes.
J. Sci. Food Agric.
Institute of Chemistry
Slovak Academy of Sciences
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2017