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Research Interests
Enzymatic synthesis of oligosaccharides; design and control of industrial biocatalysts; immobilization; and enzyme engineering.
Selected Publications (full list available on request)
Production of cytidine 5'-monophospho-N-acetyl-β-D-neuraminic acid (CMP-sialic acid) using enzymes or whole cells entrapped in calcium silica pectate gel beads.
Nahalka J., Wu B., Shao J., Gemeiner P., Wang P.J. Biotechnology and Applied Biochemistry 40: 101-106 (2004) Super-beads: immobilization in "sweet" chemistry.
Nahalka J., Liu Z., Chen X., Wang P.G. Chemistry - A European Journal 9: 373-377 (2003) Biocatalytic synthesis of UDP-GlcNAc by multiple enzymes co-immobilized on agarose beads.
Shao J., Zhang J., Nahalka J., Wang P.J. Chemical Communication 2586-2587 (2002) Nucleoside triphosphates production using recombinant E. coli entrapped in calcium pectate gel.
Nahalka J., Liu Z., Gemeiner P., Wang P.G. Biotechnology letters 24:925-930 (2002) New approaches for verification of kinetic parameters of immobilized concanavalin A: invertase preparations investigated by flow microcalorimetry.
Gemeiner P., Dočolomanský P., Nahálka J., Štefuca V., Danielsson B. Biotechnology and Bioengineering 49: 26-35 (1996) |