Molecular glycobiology with main research area in molecular cloning, expression and structure - function study of the carbohydrate processing enzymes.

Mucha J, Domlatil J, Lochnit G, Rendic D, Paschinger K, Hinterkorner G, Hofinger A, Kosma P, Wilson IB.
The Drosophila melanogaster homologue of the human histo-blood group Pk gene encodes a glycolipid-modifying alpha1,4-N-acetylgalactosaminyltransferase.
Biochem J. (2004) 15, 382:67-74

R.Griessler, A. Schwarz, J.Mucha, B. Nidetzky
Tracking interactions that stabilize the dimer structure of starch phosphorylase from Corynebacterium callunae. Roles of Arg234 and Arg242 revealed by sequence analysis and site-directed mutagenesis. Eur J Biochem. (2003)270,10:2126-36.

T. Peterbauer, U. Karner, J. Mucha, L. Mach, D. A. Jones, C. L. Hedley and A. Richter
Enzymatic control of the accumulation of verbascose in pea seeds
Plant, Cell and Environment (2003)26, 1385-1391

J.Mucha, B. Svoboda, S. Kappel, R. Strasser, P. Bencur, U. Frohwein, H. Schachter, L. Mach and J. Glossl
Two closely related forms of UDP-GlcNAc: alfa 6-D-mannoside beta 1,2-N-acetylglucosaminyltransferase II occur in the clawed frog X. laevis
Glycoconj. J. (2003), 19, 187-195

T.Peterbauer, L. Mach, J. Mucha, A. Richter
Functional expression of a cDNA encoding pea (Pisum sativum L.) raffinose synthase, partial purification of the enzyme from maturing seeds, and steady-state kinetic analysis of raffinose synthesis.
Planta. (2002)215(5):839-46

T. Peterbauer, J. Mucha, L. Mach, A. Richter
Chain Elongation of raffinose in pea seeds. Isolation, characterization, and molecular cloning of mutifunctional enzyme catalyzing the synthesis of stachyose and verbascose.
J Biol Chem. (2002) 4;277(1):194-200.

T. Peterbauer, L.B. Lahuta, A. Blochl, J. Mucha, D.A. Jones, C.L.Hedley, R.J. Gorecki, A. Richter
Analysis of the raffinose family oligosaccharide pathway in pea seeds with contrasting carbohydrate composition.
Plant Physiol. (2001)127(4):1764-72.

J. Mucha, B. Svoboda, U. Frohwein, R. Strasser, m. Mischinger, H. Schwihla, F. Altmann, W. Hane, H. Schachter, J. Gloessl, L. Mach
Tissues of the clawed frog Xenopus laevis contain two closely related forms of UDP-GlcNAc:alpha3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I.
Glycobiology. (2001)11(9):769-78

C. Breton, J. Mucha, C. Jeanneau
Structural and functional features of glycosyltransferases.
Biochimie. (2001)83(8):713-8. Review.

I.B. Wilson, D. Rendic, A. Freilinger, J. Dumic, F. Altmann, J. Mucha, S. Muller, M.T. Hauser
Cloning and expression of cDNAs encoding alpha1,3-fucosyltransferase homologues from Arabidopsis thaliana.
Biochim Biophys Acta. (2001)1527(1-2):88-96

R. Strasser, J. Mucha, L. Mach, F. Altmann, I.B. Wilson, J. Gloessl, H. Steinkellner
Molecular cloning and functional expression of beta1, 2-xylosyltransferase cDNA from Arabidopsis thaliana.
FEBS Lett. (2000)472(1):105-8.

H. Leiter, J. Mucha, E. Staudacher, R. Grimm, J. Gloessl, F. Altmann
Purification, cDNA cloning, and expression of GDP-L-Fuc:Asn-linked GlcNAc alpha1,3-fucosyltransferase from mung beans.
J Biol Chem. (1999)274(31):21830-9.