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Research Interests
Development, characterization and optimization of novel immobilized biocatalysts for enantioselective,
environment-friendly and economical production of bioactive compounds: encapsulation of cells/enzymes
in polymer microcapsules with controllable functional properties; development of efficient
biotechnological processes in laboratory scale utilizing encapsulated cells/enzymes.
Publications
Hucík, M., Bučko, M., Gemeiner, P., Štefuca, V., Vikartovská, A., Mihovilovič, M.D., Rudroff, F., Iqbal, N., Chorvát, D. Jr., Lacík, I.
Encapsulation of recombinant E. coli expressing cyclopentanone monooxygenase in polyelectrolyte complex capsules for Baeyer-Villiger biooxidation of 8-oxabicyclo[3.2.1]oct-6-en-3-one. Biotechnol. Lett., accepted
Bučko, M., Gemeiner, P., Vikartovská, A., Mislovičová, D., Lacík, I., Tkáč, J.
Coencapsulation of oxygen carriers and glucose oxidase in polyelectrolyte complex capsules for the enhancement of D-gluconic acid and δ-gluconolactone production. Artif. Cells Blood Substit. Biotechnol., accepted
De Vos, P., Bučko, M., Gemeiner, P., Navrátil, M., Švitel, J., Faas, M., Strand, B.L., Skjak-Braek, G., Morch, Y.A., Vikartovská, A., Lacík, I., Kolláriková, G., Orive, G., Poncelet, D., Pedraz, J.L., Ansorge-Schumacher, M.B.
Multiscale requirements for bioencapsulation in medicine and biotechnology. Biomaterials 30, 2559-2570 (2009)
Šefčovičová, J., Katrlík, J., Štefuca, V., Mastihuba, V., Voštiar, V., Greif, G., Bučko, M., Tkáč, J., Gemeiner, P.
A filtration probe-free on-line monitoring of glycerol during fermentation by a biosensor device. Enzyme Microb. Technol. 42, 434-439 (2008)
Prüsse, U., Bilancetti, L., Bučko, M., Bugarski, B., Bukowski, J., Gemeiner, P., Lewinska, D., Manojlovic, V., Massart, B., Nastruzzi, C., Nedovic, V., Poncelet, D., Siebenhaar, S., Tobler, L., Tosi, A., Vikartovská, A., Vorlop, K.-D.
Comparison of different technologies for alginate beads production. Chem. Pap. 62, 364-374 (2008) PhD thesis:
Bučko, M.:
Immobilized biosystems: Potential and limitations of encapsulation of biocatalysts in polymer microcapsules (Imobilizované biosystémy: Potenciál a obmedzenia enkapsulácie biokatalyzátorov v polymérnych mikrokapsuliach) Institute of Chemistry, Slovak Academy of Sciences, Bratislava, 2008
Vikartovská, A., Bučko, M., Mislovičová, D., Pätoprstý, V., Lacík, I., Gemeiner, P.
Improvement of the stability of glucose oxidase via encapsulation in sodium alginate-cellulose sulfate-poly(methylene-co-guanidine) capsules. Enzyme Microb. Technol. 41, 748-755 (2007)
Švitel, J., Tkáč, J., Voštiar, I., Navrátil, M., Štefuca, V., Bučko, M., Gemeiner, P.
Gluconobacter in biosensors: Applications of whole cells and enzymes isolated from Gluconobacter and Acetobacter to biosensor construction. Biotechnol. Lett. 28, 2003-2010 (2006)
Nahálka, J., Gemeiner, P., Bučko, M., Wang, P.G.
Bioenergy beads: A tool for regeneration of ATP/NTP in biocatalytic synthesis. Artif. Cells Blood Substit. Biotechnol. 34, 515-521 (2006)
Mislovičová, D., Masárová, J., Bučko, M., Gemeiner, P.
Stability of penicillin G acylase modified with various polysaccharides. Enzyme Microb. Technol. 39, 579-585 (2006)
Bučko, M., Vikartovská, A., Gemeiner, P., Lacík, I., Kolláriková, G., Marison, I.W.
Nocardia tartaricans cells immobilized in sodium alginate-cellulose sufate-poly(methylene-co-guanidine) capsules: mechanical resistance and operational stability. J. Chem. Technol. Biotechnol. 81, 500-504 (2006) My Diploma Thesis:
Špirková, K., Bučko, M., Stankovský, Š.
Imidoyl isothiocyanates in the synthesis of condensed heterocycles: preparation of some substituted benzotriazocines. Arkivoc 2005, Part(v), 96-102 (2005)
Bučko, M., Vikartovská, A., Lacík, I., Kolláriková, G., Gemeiner, P., Pätoprstý, V., Brygin, M.
Immobilization of a whole-cell epoxide-hydrolyzing biocatalyst in sodium alginate-cellulose sulfate-poly(methylene-co-guanidine) capsules using a controlled encapsulation process. Enzyme Microb. Technol. 36, 118-126 (2005)
Vikartovská, A., Bučko, M., Gemeiner, P., Nahálka, J., Pätoprstý, V., Hrabárová, E.
Flow calorimetry - a useful tool for determination of immobilized cis-epoxysuccinate hydrolase activity from Nocardia tartaricans. Artif. Cells Blood Substit. Biotechnol. 32, 77-89 (2004) |